Sir J J Hospital

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Para-clinical Department Back to Departments
Name of the department	Department of Microbiology
Address	Pathology School (1st Floor) – Bacteriology, Hospital Infection Surveillance, Mycology & Mycobacteriology Laboratories Pathology School (2nd Floor) – Office, Academic Section & Media Preparation Laboratory 2nd Floor of Skin & VD Department – ICTC, Serology & Immunology Laboratories
Phone Numbers	Microbiology Direct – 23712454 ICTC, Serology, Immunology Direct – 23710234 J.J. Hospital Board Number – 23735555 Extension – 2359 (Microbiology - 2nd Floor) Extension – 2276 (1st Floor Laboratories) Extension – 2351 (ICTC, Serology, Immunology Laboratories)
Fax Numbers	022-23735599
Email Address	microbiologygmc@gmail.com


About the department-

Name	Designation	Mobile Number	Email-ID
Dr. Ameeta Joshi	Head of the Department & I/C ICTC	9323824397	ameeta.joshi@gmail.com
Dr. Neeta Jangale	Associate Professor & I/C Serology & Immunology	9869403485	dr_neetaj@hotmail.com
Dr. Chhaya Chande	Associate Professor & I/C Bacteriology & Media Preparation Laboratory	9823056377	cachande@gmail.com
Dr. Sarala Menon	Associate Professor & I/C Mycology, Mycobacteriology & Parasitology	9867789568	saralagopalmenon@yahoo.co.in
Dr. Shilpa Patil	Associate Professor & I/C Academics	9321970777	shilpashaileshpatil@gmail.com
Dr. Shubhra Sengupta	Lecturer	9869318799	shubhrasengupta9@yahoo.co.in
Mrs. Saroj Turbadkar	Lecturer	9820944342	sarojdt@yahoo.co.in
Dr. Varsha Wanjare	Lecturer & I/C Hospital Infection Surveillance Laboratory	9224384504	shashirwanjare@yahoo.co.in
Dr. Nilma Hirani	Lecturer	9820556255	nbhirani@yahoo.com
Mrs. Vaishali Wabale		9321486661	rwabale@gmail.com
Dr. Sunil Lilani		9322299008	lilanisunil@gmail.com
Dr. Shazia Chavan		9820638948	shazia14@rediffmail.com
Dr. Ritesh Shirpurkar		9323196573	sh_ritesh@yahoo.com
Dr. Monita Kulkarni		9322952893	kulkarnimonita@yahoo.com
Dr. Pradnya Veer		9821946221	----
Dr. Kavita Chopdekar		9324696573	spad_bhilkar@rediffmail.com

SERVICES PROVIDED

Microbiology Department is a Laboratory Medicine Department, functioning at Sir J.J. Hospital, Mumbai. Dr. Ameeta Joshi heads this Department. We are involved with processing samples for Microbiological Investigations. There are various sections in our laboratory, where processing of samples is done to help clinicians come to a diagnosis.

The various sections of our laboratory & their statistics for the year 2008-2009 are as follows:

NAME OF THE LABORATORY	TOTAL SAMPLES PROCESSED IN YEAR 2008
Bacteriology Laboratory
Bacterial Cultures	17605
Emergency Services	2505
Hospital Infection Surveillance Laboratory	5025
MYCOBACTERIOLOGY LABORATORY
AFB slides Screened	1468
AFB Cultures	1078
MYCOLOGY LABORATORY
Fungal culture	900
ICTC
HIV Testing	20537
Clients Counseled	7001
CD4 Testing	19277
SEROLOGY & IMMUNOLOGY LABORATORY
Widal	1376
VDRL	6449
ASO	739
CRP	2042
RA	957
HBsAg	14820
Detection of IgM & IgG antibodies to Dengue virus	1018
Malaria antigen detection	120
Leptospira IgM & IgG antibody detection	1003

SERVICES PROVIDED

A. BACTERIOLOGY LABORATORY:

Routine laboratory services – bacterial culture & sensitivity on pus, blood, urine, sputum, stool, body fluids, CSF
Emergency services – Microscopy & bacterial culture of specimens received for emergency testing

B. HOSPITAL INFECTION SURVEILLANCE LABORATORY:

Regular Hospital Rounds
Operation Theatre Surveillance (J.J. Hospital, G.T. Hospital, St. George Hospital & CAMA Hospital)
Hospital Infection Policy Formulation
Outbreak Investigation
Regular HIC & BMW Sensitization Workshops

C. PARASITOLOGY LABORATORY:

Saline & Iodine mount of stool samples
Modified Z-N staining for parasites

D. MYCOBACTERIOLOGY LABORATORY:

Ziehl Neelsen staining – 25%, 5%, 1% & Fluorescent Microscopy
Culture of Mycobacteria
Anti-tuberculosis Drug Sensitivity Testing by Proportion method for first line drugs (INH, Streptomycin, Rifampicin & Ethambutol)

E. MYCOLOGY LABORATORY:

Fungal Cultures
India Ink Preparation
Culture of Cryptococci
Giemsa & GMS staining for Pneumocystis carinii

F. SEROLOGY & IMMUNOLOGY LABORATORY:

Widal
VDRL
TPHA
ASO
CRP
RA
HBsAg
HCV for antibodies
Detection of IgM & IgG antibodies to Dengue Virus
Malaria antigen detection
Leptospira IgM & IgG antibody detection
TORCH panel
HAV, HEV
Chikungunya
Japanese Encephalitis

G. ICTC:

HIV antibody testing of Indoor patients
Referrals for NGO’s, CBO, ART Centre, RNTCP, PPTCT, DIC of HIV sero-positive patients as per need
CD4 testing of HIV sero-positive patients via ART Center

H. ACADEMIC SECTION:

Apart from being a Diagnostic laboratory, we are also involved with the Academic activities. The Academic Section deals with the smooth functioning of the teaching activities conducted by the Department which includes

preparing time tables
Lectures, tutorials & practicals
Examinations
Internal Assessments
Organization of Seminars
Organization of Quiz
Feedback from students on regular basis
Organization of workshops for MSc. Microbiology & Biotechnology students
Teaching of OT Technicians of P.D. Hinduja Management studies
Coordination of all academic activities

COURSES OFFERED WITH INTAKE CAPACITY
Courses Taught	Capacity of students every year (approx.)
PG students (MD Microbiology)	2
MBBS	200
BDS	100
Post-Basic BSc Nursing	30
Basic BSc Nursing	50
Pediatric Nursing	30
General Nursing	50
DMLT	12
In-Service Technicians	10

EQUIPMENTS AVAILABLE:

NAME OF THE EQUIPMENT

Refrigerator
Laboratory Refrigerator
Deep Freeze
Walk in cooler
CO₂ incubator
Incubator
Microscope – Monocular
Microscope – Binocular
Fluorescent microscope
Research microscope
GasPak system
Biosafety Cabinet – Class II
Inspissator
Centrifuge
Bottle Centrifuge
Refrigerated Centrifuge
Water bath
Hot Air Oven
Autoclave
Micropipettes with varying calibrations
ELISA system
Glass Distillation Apparatus
VDRL Rotator
FACS Calibur CD4 Testing Machine with its paraphernalia: blood rocker, vortex machine, computer with printer
Slide projector
Overhead Projector
Computers with printers

PUBLICATIONS:

ALREADY PUBLISHED:

SR. NO.	TITLE OF PUBLICATION	PRINCIPAL AUTHOR	JOURNAL	YEAR & DETAILS
01.	Current Epidemiology of Intracranial Abscesses: A prospective five years study & literature review	Dr. Saral Menon	Journal of Medical Microbiology (U.K.)	Vol. 57, 1259 - 1268
02.	An insight into human cryptosporidiosis	Dr. Neeta Jangale	Knowledge Update Program Technical report Series(MUHS Nashik)	---
03.	Disinfectants – Facts & Findings	Dr. Neeta Jangale & Dr. Abhay Chowdhary	Newsletter of Hospital Infection Society Mumbai Forum	---

PUBLICATIONS IN PROCESS:

Sr. No.	TITLE OF PUBLICATION
01.	Study of Carbapenem Resistance in Pseudomonas aeruginosa
02.	Bacterial Infection Profile in Critical Care Units of J.J. Hospital
03.	Salmonella typhimurium infection in children: An overview
04.	Effect of Sensitization of BMW workshop on HCW at J.J. Hospital
05.	Biomedical Waste Audit
06.	Neurosyphilis – A case report

PRESENTATIONS:

Sr. No.	TITLE OF PRESENTATION	PRINCIPAL AUTHOR	PLACE OF PRESENTATION	YEAR & DETAILS
01.	Chromoblastomycosis & Pheaohyphomycosis: Two case reports	Dr. Sarala Menon	KEM Hospital, Mumbai	SIHAM 2008
02.	Capture ELISA vis-à-vis Immunochromatography for Dengue diagnosis	Dr. Nilma Hirani	National Conference of IAMM, Pune	October 2008
03.	Neurosyphilis – a case report	Dr. Susheela Chaurasia	National Conference of IAMM, Pune	October 2008
04.	Biomedical Waste Audit	Dr. Varsha Wanjare	2nd HIS National Conference	March 2009
05.	Carbapenem Resistance in Clinical isolates of Pseudomonas aeruginosa at a tertiary care Hospital	Dr. Shazia Chavan	2nd Infectious Diseases National conference	March 2009
06.	HBV & HCV Co-infection in HIV sero-positive patients	Mr. Saumil Pandya Guide- Dr. Neeta Jangale	Sent for MEDICON – ICMR Conference	June 2009

RESEARCH PROJECTS – COMPLETED:

Sr. No.	TITLE OF PUBLICATION	AUTHORS	LABORATORY WHERE WORK WAS CARRIED OUT	YEAR & DETAILS
01.	A study of prevalence of MRSA in Dermatology ward of Tertiary Care Hospital, Mumbai	Ms. Ramanika Gumber (MBBS student) Dr. Shazia Chavan	Bacteriology	ICMR project 2008
02.	Pre & Post test evaluation of HCW on BMW at J.J. Hospital	Dr. Varsha Wanjare	HIS	2008
03.	Biomedical Waste Audit	Dr. Varsha Wanjare	HIS	March 2009
04.	Biofilm production in Candida isolated from different clinical specimens	Mr. Saurabh Muni (MBBS student) Dr. Sarala Menon – Guide	Mycology	ICMR project
05.	Co-existence of Candida species in pulmonary tuberculosis patients	Ms. Khushboo (MBBS student) Dr. Sunil Lilani Guide	Mycology	ICMR Project
06.	Mycological profile of infections in patients attending Skin OPD in a tertiary Care Centre	Mr. Yogesh Sawakare (MBBS student) Dr. Sunil Lilani - Guide	Mycology	ICMR Project
07.	Serological Evaluation of PUO in a Tertiary Care Hospital	Mr. Sagar Mehta (MBBS student) Dr. Nilma Hirani - Guide	Serology	ICMR Project
08.	HBV & HCV Co-infection in HIV sero-positive patients	Mr. Saumil Pandya Guide- Dr. Neeta Jangale	Serology	ICMR Project
09.	A study of prevalence of metalo beta lactamase producing Pseudomonas aeruginosa at Sir J.J. hospital, Mumbai	Mr. Rahul Mali (MBBS student), Dr. Shazia Chavan - Guide	Bacteriology	MUHS STRS Project

RESEARCH PROJECTS – ON-GOING:

Sr. No.	TITLE OF PUBLICATION	LABORATORY WHERE WORK IS BEING CARRIED OUT	YEAR
01.	Study of Vancomycin Susceptibility by MIC in Enterococcus faecalis	Bacteriology	2009
02.	Characterization of MRSA isolates	Bacteriology	2009
03.	Study of prevalence & antibiotic susceptibilities of Acinetobacter baumanii	Bacteriology	2009
04.	A study of prevalence of Metalo beta lactamases producing Pseudomonas aeruginosa at Sir J.J. Hospital, Mumbai	Bacteriology	2009
05.	Evaluation of laboratory & Clinical parameters for diagnosis of PICU bloodstream infections	Bacteriology	ICMR 2009
06.	Characterization of MRSA from clinical isolates	Bacteriology	ICMR 2009
07.	In-Use Testing of Disinfectants	HIS	2009

POST-GRADUATE DISSERTATION:

Sr. No.	DISSERTATION TOPIC	NAME OF POST-GRADUATE STUDENT	NAME OF THE GUIDE	YEAR
01.	Evaluation of different laboratory tests in the diagnosis of leptospirosis	Dr. Charuta Date	Dr. Ameeta Joshi	January 2008
02.	Study of etiopathogenesis of parasitic diarrhea in people living with HIV/AIDS	Dr. Nishat Hussain	Dr. Abhay Chowdhary	May 2008
03.	Blood stream infections in Intensive Care Units	Dr. Swapna Mali	Dr. Neeta Jangale	November 2008
04.	Evaluation of protective antibodies in Hepatitis B Vaccinated Health Care Workers	Dr. Majid Khan	Dr. Abhay Chowdhary	--
05.	Laboratory Diagnosis of Non-tuberculous meningitis in HIV Positive patients	Dr. Susheela Chaurasia	Dr. Ameeta Joshi	--
06.	Prevalence of IgM & IgG antibodies against TORCH infection in patients with BOH	Dr. Jyoti Bade	Dr. Varsha Wanjare	--
07.	Comparison of different methods for diagnosis of malaria	Dr. Ashwini Waghmare	Dr. Abhay Chowdhary	--
08.	Opportunistic Fungal Infections in HIV patients	Dr. Saalim	Dr. Chhaya Chande	--

TRAINING PROGRAMS:

TRAINING PROGRAM CONDUCTED	YEAR & DETAILS
HIC & BMW Workshops in Wards, OT, ICU’s & for General staff	2008 throughout the year
Current Practices in Serology- Immunology	23^rd September to 1^st October 2008
Training of labor ward nurses in HIV testing & Counseling during emergency	18^th February 2008
Training Workshop in Summer & Diwali Vacation for MSc. Microbiology students & Biotechnology students	2008 ( Summer & Diwali vacation)
Training of Laboratory Technicians for EQAS program	7^th August 2008 & 26^th March 2009
Refresher training of ICTC Laboratory Technicians	17 – 19^th March 2009

GUEST LECTURES:

Sr. No.	TITLE OF PUBLICATION	SPEAKER	VENUE	YEAR
01.	In-Vitro Spectroscopy	Dr. Sarala Menon	Neurosurgery Golden Jubilee Celebrations of Sir J.J. Hospital	28 April 2008
02.	Universal Safety Precautions & Biomedical waste Management lectures for Interns	Dr. Varsha Wanjare	J.J. Hospital	January 2009
03.	Universal Safety Precautions & Biomedical waste Management lectures for HCW	Dr. Varsha Wanjare	Bacchu Eye Hospital	January 2009
04.	Universal Safety Precautions & Biomedical waste Management lectures for ICTC Technicians	Dr. Varsha Wanjare	Basic training ICTC at Nair Hospital, Mumbai	March 2009
05.	Guest speakers for various HIV related workshops organized by MDACS, MSACS, ART Centre, PPTCT Centre & ICTC at Sir J.J. Hospital	Dr. Ameeta Joshi Dr. Neeta Jangale Dr. Nilma Hirani Dr. Ritesh Shirpurkar	J.J. Hospital	2008 - 2009
06.	Antibiotic Resistance: J.J. Hospital Scenario & Rational Use of Antibiotics	Dr. Chhaya Chande	Department of Surgery, J.J. hospital, Mumbai	March 2009

AWARDS & MERITS:

Sr. No.	TITLE OF PUBLICATION	AUTHOR	AWARD/ MERIT	YEAR & DETAILS
01.	Best ICTC – year 2007
02.	Microphotograph Contribution - This image appeared on cover-page of this issue	Dr. Sarala Menon & Ms. Aruna Gohil	Journal of Medical microbiology, Oct 2008, Vol. 57.	October 2008
03.	Ms. Aruna Gohil	--	Best Technician Award	26 January 2009
04.	Dr. Swapna Mali	--	Best Post-graduate student	26 January 2009
05.	Comparison of Fluorescent Microscopy & Z-N staining for detection of Mycobacterium tuberculosis in pulmonary tuberculosis patients	Mr. Dhrumin Sangoi (MBBS student) Mrs. Sujata Dharmashale (Guide)	Winner of ICMR Short Term Research Studentship	March 2009
06.	Ghrapure Award, Gold medal & Rank Certificate for MD Microbiology MUHS University Examination – Dr. Nishat Hussain			2009

FUTURE PLANS:

Sr. No.	DETAILS
01.	Mycobacteriology Laboratory is getting accredited for culture & drug sensitivity testing under RNTCP – Centre identified for DOTS Plus Program
02.	Booklets & Guidelines on HIC & BMW, MET
03.	Arranging workshops for Surgeons, Physicians & Intensivists separately for HIC & BMW
04.	Accreditation of Academic Section, CD4 laboratory
05.	Accreditation of CD4 laboratory
06.	To start Integrated teaching Program
07.	Microbial repository

EQUIPMENTS UNDER PMSSY:

NAME OF EQUIPMENT	SERVICES THAT WILL BE PROVIDED
Automated blood culture system	Awaited – for rapid diagnosis of bacteria
Automated mycobacterial culture system	Awaited – for rapid diagnosis of bacteria
Real Time PCR	Diagnosis of Infectious diseases especially: HIV, HCV, STI, leptospira, Dengue, Malaria etc.
Lyophilizer	Microbial repository
Deep Freeze - 86°C	For storing viral cultures

INFORMATION TO PATIENTS:

Information to patients: ICTC

ICTC stands for Integrated Counseling & Testing Centre (for HIV)
Location – 2nd Floor, Skin & STD Building
Timings: 9.30 a.m. to 4.30 p.m.
Counseling & Testing of Direct walk-in clients done free of charge
Blood collection next to counselors room
IEC material at hand – brochures / pamphlets free
TV/ DVD in waiting area
Condom – pick-up in waiting area
Reports of HIV test available same day
CD4 count testing available to patients who are registered at ART center, Sir J.J. Hospital, No charges
Road map

USEFUL LINKS:

IMPORTANT ANNOUNCEMENT:

XV^th Maharashtra State Chapter Conference of Indian Association of Medical Microbiologists (MICROCONF 2009) to be hosted by the Department on 12^th & 13^th of September 2009, preceded by CME on the 11^th September 2009.

Theme of the CME: Resistance in Tuberculosis
Theme of Conference: Microbial Drug Resistance

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Theme of Conference: Microbial Drug Resistance